Microscopy Workshops
Overview — The Center
for Plant Cell Biology offers microscopy tutorials to groups of four to ten people
on subjects such as those listed below. Please contact David
Carter to schedule a training session for your group, or to arrange to join
a group. Training will typically take place on Wednesday mornings between 9am
and 12pm, and involve both a theory presentation and hands-on demonstration. Tutorials
can usually be scheduled two weeks after the request is received, depending on
demand. Back to Top 
2006 Schedule
of Workshops — (Noel Keen Hall is the north wing of Batchelor Hall)
| Date | Day | Time | Title
of Session | Location | Limit
Per Session |
| July 19, 2007 |
Thurs |
9am-12pm |
Atto Pathway HT High Throughput Confocal Imaging
This workshop is suitable for anyone interested in collecting camera-based confocal images with UV and visible dyes, including GFP expression in live animal cells or whole plant seedlings. The system is well suited to many kinds of automated imaging and quantitation, including low magnification and high resolution montages, extended time course experiments and large screening experiments, including high throughput chemical and mutant screens. Feedback from this workshop will be used to develop future capabilities for this highly versatile automated system.
|
Noel Keen Hall, Rm 2018 |
6 people |
| June 28, 2007 |
Thurs |
9am-12pm |
Zeiss 510 Confocal Training
This workshop is intended for those new to confocal microscopy, as well as for users who need an alternative to the more heavily used Leica SP2 confocal system. This system collects top quality images and has extensive visualization and 3D rendering capability. The session starts with about 90 minutes of detailed theory, followed by 90 minutes of hands-on practice. Users are welcome to bring their own samples, although there may not be time to look at them.
|
Noel Keen Hall, Rm 2018 |
10 people |
| May 17, 2007 |
Thurs |
9am-12pm |
Leica SP2 Confocal Training
This workshop is suitable for first time confocal users and for people cross-training from the Zeiss, Pathway or Meridian confocals. The second half of the session will be hands on practice, so you may bring your own samples in case there is time to look at them. Training is free, but a valid FAU number is required for billing of future instrument use.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Apr. 26, 2007 |
Thurs |
10am-1pm |
Leica SP2 Confocal Training
This workshop is suitable for first time confocal users and for people cross-training from the Zeiss, Pathway or Meridian confocals. The second half of the session will be hands on practice, so you may bring your own samples in case there is time to look at them.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Apr. 12, 2007 |
Thurs |
11am-1pm |
Hitachi TM-1000 Tabletop Scanning Electron Microscopy Training
This new type of scanning electron microscope is fast and intuitive, and requires no sample preparation! The software is as easy to use as a digital camera, and creates 1024x960 images at up to 10,000x magnification. The workshop will cover all aspects of using the instrument, including how to handle samples, how to measure features of interest, and how to freeze wet samples for extended viewing time.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Apr. 11, 2007 |
Wed |
9am-1pm |
Zeiss 510 Confocal Training
The Zeiss 510 confocal is our highest resolution system, with similar capabilities to the heavily used Leica SP2. Users of the SP2 who do not require UV or 442 laser lines are encouraged to cross train on the Zeiss. The workshop will cover basic imaging and 3D z-series collection. The new pulsed dye laser addition to this system will also be described. |
Noel Keen Hall, Rm 2018 |
10 people |
| Mar. 21, 2007 |
Wed |
10am-1pm |
Leica SP2 Confocal Training
This workshop is suitable for first time confocal users and for people cross-training from the Zeiss, Pathway or Meridian confocals. The second half of the session will be hands on practice, so you may bring your own samples in case there is time to look at them. Training is free, but a valid FAU number is required for billing of future instrument use.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Feb. 28, 2007 |
Wed |
10am-1pm |
Leica SP2 Confocal Training
This workshop is suitable for first time confocal users and for people cross-training from the Zeiss, Pathway or Meridian confocals. The second half of the session will be hands on practice, so you may bring your own samples in case there is time to look at them. Training is free, but a valid FAU number is required for billing of future instrument use. |
Noel Keen Hall, Rm 2018 |
10 people |
| Jan. 30, 2007 |
Tues |
1-3pm |
MMI CellCut Workshop
Laser Capture Micro-dissection demonstration. This system boasts the ability to cut paraffin sections, cryo sections, smears and even cell cultures with zero contamination, for isolating individual cell types from mixed tissues and cultures for molecular analysis. The instrument will be running all afternoon and all day Wednesday January 31st for evaluation.
If your lab is interested in having access to this technology, your participation is needed. Will it work on your experimental system? How much use will it get if it is purchased for the core facility? If you are interested in isolating some cells during this demonstration, please contact David Carter at dcarter@ucr.edu for plasticware and protocols.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Jan. 25, 2007 |
Thurs |
9am-12pm |
Leica SP2 Confocal Microscope
For first-time confocal users and for cross-training from other confocal instruments. This instrument is easy to use and gives the highest resolution. It has 9 laser lines for excitation and unlimited selection of detection wavelength range. The second half of the session will be hands on practice, so bring your own samples. Training is free, but you need to provide a valid FAU number for future instrument use.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Dec. 19, 2006 |
Tues |
12pm |
Zeiss PALM Laser Capture Micro-dissection Demonstration
Laser capture micro-dissection enables users to isolate a single cell type from tissue sections for more precise genomic or proteomic investigation. PALM is one of the leading brands for this technology and works on cryosections, vibratome sections, wax sections and can even pull live cells out of a mixed population in custom dishes. A 20 minute slide presentation and discussion will be followed by hands-on demonstration in room 1005 downstairs. The PALM system will be running all afternoon, so feel free to bring samples.
|
Noel Keen Hall, Rm 2018 |
|
| Nov. 29, 2006 |
Wed |
9am-12pm |
Zeiss 510 Confocal Microscope Training
The Zeiss 510 is a premium quality point scanning visible light confocal system on an automated upright microscope stand, which has just been fitted with a MicroPoint dye laser for ablation and photobleaching. This workshop is suitable for first time confocal users and those cross-training from the Leica, Pathway or Meridian confocals. The second half of the session will be hands on practice, so feel free to bring your own samples.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Aug. 22, 2006 |
Tues |
TBD |
Electron Microscope
This instrument can collect images of unprepared samples, including TEM grids, and has a resolution of about 25 nm. If you are interested in looking at a new scanning electronic microscope which is available for demo, please contact David Carter at dcarter@ucr.edu. |
TBD |
TBD |
| Aug. 9, 2006 |
Wed |
9am-1pm |
Pathway HT High Throughput Automated Confocal Microscope
This system is ideal for all kinds of high throughput and extended time course experiments. It also has montage and kinetics imaging capability. New software on this high throughput automated microscope will make routine imaging easier and facilitate more advanced imaging and analysis.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Aug. 2, 2006 |
Wed |
9am-11pm |
Leica SP2 Confocal Microscope
This system produces the best quality confocal images, has the most lasers (9) and the widest range of emission range detection (4 fully configurable channels plus transmission). It is also easy to use for routine imaging. This workshop is for you if you use a fluorescence microscope but sometimes need higher quality images for publications, or if you want to start using a confocal microscope.
|
Noel Keen Hall, Rm 2018 |
10 people |
| May 3, 2006 |
Wed |
9am-12pm |
Zeiss 510 Confocal Microscope
This workshop is intended for those new to confocal microscopy, as well as for users who need an alternative to the more heavily used Leica SP2 confocal system. This system collects top quality images and has extensive visualization and 3D rendering capability.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Apr. 26, 2006 |
Wed |
9am-12pm |
Pathway HT High Throughput Automated Confocal Microscope
This system is ideal for all kinds of high throughput and extended time course experiments. It also has montage and kinetics imaging capability. New software on this high throughput automated microscope will make routine imaging easier and facilitate more advanced imaging and analysis.
|
Noel Keen Hall, Rm 218 |
10 people |
| Apr. 20, 2006 |
Thurs |
9am-12pm |
Biomek FX Fluid Handling Robot
To support chemical library management and all kinds of fluid handling applications, we have added a Biomek FX fluid handling robot to the microscopy core facility. This system has 96 tip dispensing capability, 96 pin fluid transfer, a gripper for moving plastic ware around and a span-8 head for single well dispensing. Anyone with a specific application should contact David Carter well in advance.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Apr. 12, 2006 |
Wed |
9am-12pm |
Yokogawa Confocal Microscope
To support high speed kinetics imaging and high throughput screening, we have added a Yokogawa spinning disc confocal system to the microscopy core. This system has the new EMCCD camera technology for extreme sensitivity, and motorized stage for assisted navigation and montage capability.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Apr. 6 , 2006 |
Thurs |
9am-12pm |
Zeiss 510 Confocal Microscope
This workshop is intended for those new to confocal microscopy, as well as for users who need an alternative to the more heavily used Leica SP2 confocal system. This system collects top quality images and has extensive visualization and 3D rendering capability.
|
Noel Keen Hall, Rm 2018 |
10 people |
| Mar. 29, 2006
|
Wed |
9am-12pm |
Leica
SP2 Confocal Microscope
This system produces the best quality confocal images, has the most lasers (9) and the widest range of emission range detection (4 fully configurable channels plus transmission). It is also easy to use for routine imaging. This workshop is for you if you use a fluorescence microscope but sometimes need higher quality images for publications, or if you want to start using a confocal microscope.
|
Noel Keen Hall, Rm
2018 |
10 people |
Back
to Top Who
Should Attend — These sessions are intended for scientists who
want to use the resources of the Noel T. Keen Hall Facility in their research.
Training will be sufficient to get you started on using the featured equipment,
and top-up training will be provided at no extra charge during the first few booked
usage sessions. At present, microscopy instrumentation in this facility consists
of: - (4) Confocal Microscopes
- (2) Dissection Microscopes
- (1) Fluid Handling Robot
- (1)
Laser Ablation System
- (1) Vibrating Microtome
- (2) Ultra Microtome
- (1)
Image Analysis Station
- (1) Cryostat
- (1) Gene Gun
Back
to Top How
To Register —
To register for the workshops, please email David
Carter, or call 951/827-2694 (x2-2694). You will need to provide the name of
the lab PI, email address and the complete FAU numbers for billing purposes
[for future use of equipment. Please also review Instructions
to UCR Personnel for Online Microscopy Booking System (PDF)]. Make sure to
indicate any particular experimental
problem or question you would like to see addressed.
Back
to Top Location
and Tours of the Noel T. Keen Hall —
The Noel T. Keen Hall is located
in the newly-renovated, 10,000 square-foot north wing of Batchelor Hall. For precise
directions, visit Keen Hall on the UCR campus map. Keen Hall is a shared-use facility providing instrumentation
suites in microscopy and imaging, bioinformatics, proteomics, and DNA sequencing
and molecular biology.
Anyone interested in an informational tour of the
imaging facilities is welcome to contact David
Carter to arrange a time for a walk-through and discussion of what is available. Back
to Top |
